Temperature-regulated expression of the tac/lacl system for overproduction of a fungal xylanase in Escherichia coli

Temperature-regulated expression of recombinant proteins in the tac promoter (Ptac) system was investigated. Expression levels of fungal xylanase and cellulase from N. patriciarum in E. coli strains containing the natural lacI gene under the control of the Ptac markedly increased with increasing cultivation temperature in the absence of a chemical inducer. The specific activities (units per milligram protein of crude enzyme) of the fungal xylanase and cellulase produced from recombinant E. coli strain pop2136 grown at 42 degrees C were about 4.5 times higher than those of the cells grown at 23 degrees C and were even slightly higher when compared with cells grown in the presence of the inducer isopropyl beta-D-thiogalactopyranoside. The xylanase expression level in the temperature-regulated Ptac system was about 35% of total cellular protein. However, this system can not be applied to E. coli strains containing lacIq, which confers over production of the lac repressor, for high-level expression of recombinant proteins. In comparison with the lambda PL system, the Ptac-based xylanase plasmid in E. coli pop2136 gave a considerably higher specific activity of the xylanase than did the best lambda PL-based construct using the same thermal induction procedure. The high-level expression of the xylanase using the temperature-regulated Ptac system was also obtained in 10-litre fermentation studies using a fed-batch process. These results unambiguously demonstrated that the temperature-modulated Ptac system can be used for overproduction of some non-toxic recombinant proteins.     

Update rates and fidelity in virtual environments

Interaction is the primary characteristic of a Virtual Environment and update rate is normally taken as an index or measure of the interactivity of the system. The speed of many systems is dictated by the slowest component which is often the Computer Image Generator (CIG). It is common for the workload of the CIG to vary and hence the performance of the system. This paper shows how a variable update rate can produce undesirable results. Two solutions to this problem are presented: service degradation and worst-case. In the case of the CIG, service degradation would require the quality of the image to be reduced such that the time taken never exceeds a given deadline. The worst-case technique works by finding the longest time taken to render any view and then uses that as the deadline for completion. The support of predictive methods is one of several benefits of this approach. An implementation of the worst-case technique is described which takes finer control over the CIG than usual and may be applied to many existing systems with little modification.     

Phase I study of intraperitoneal metalloproteinase inhibitor BB94 in patients with malignant ascites

This was an open Phase I study of i.p. matrix metalloproteinase inhibitor BB94 in patients with malignant ascites. The objective of the study was to determine the effect of increasing i.p. doses of BB94 with reference to the tolerance, safety, and pharmacokinetics of the compound. Twenty-three patients with malignant ascites had BB94 instilled into the peritoneal cavity after paracentesis. The compound was well tolerated; no serious adverse events were seen, and no specific toxicities were observed. High plasma concentrations were seen an hour after dosing, and BB94 was still present in the plasma at day 28 after treatment at levels in excess of the IC50 identified in preclinical studies. Five of the 23 patients neither reaccumulated ascites nor died up to 112 days after dosing. Seven patients died without reaccumulating ascites. Although the study was not designed to demonstrate clinical efficacy, the results were encouraging and support the further therapeutic evaluation of matrix metalloproteinase inhibitors in the management of malignant ascites.     

A composite silent corticotroph pituitary adenoma with interspersed adrenocortical cells: case report

Recently, perfusion imaging has been of increasing interest in MRI. We applied this method for semiquantitative evaluation of hepatic parenchymal portal blood flow in patients with diffuse liver damage. Twenty patients with diffuse hepatic damage were divided according to the Child's Classification and studied. Gadolinium-diethylenetriamine pentaacetic acid (Gd-DTPA) was administered into the superior mesenteric artery (SMA), and a dynamic series of T2*-weighted fast low angle shot (FLASH) images was obtained. We evaluated relative regional portal blood volume (rrPBV), mean transit time (MTT), and relative regional portal blood flow (rrPBF). The relationship between the rrPBV, rrPBF, and plasma indocyanine green retention rate test at 15 minutes (ICGR15 was also evaluated in 12 patients. Both rrPBF and rrPBV are significantly decreased in Child B & C patients compared with Child A patients. On the other hand, the MTT is significantly prolonged in Child B & C patients compared with Child A patients. Significant correlations were also noted between rrPBV and ICGR15 and between rrPBF and ICGR15. By means of selective catheterization into the SMA, we were able to estimate rrPBV, rrPBF, and MTT. This method may play a clinical role for assessment of regional portal perfusion in various diseases with diffuse liver damage.     

Regulation of fibroblast-induced collagen gel contraction by interleukin-1beta

Renal agenesis is thought to result from a lack of induction of the metanephric blastema by the ureteral bud. Bilateral renal agenesis/dysgenesis (BRA/D) is rare, occurring in only one or two per 10,000 births. Despite its being moreover a sporadic event, there is a male to female ratio of 2.5 to 1 and approximately 20-36% of BRA/D present a familial recurrence, for genetic transmission most probably autosomal dominant with incomplete penetrance and variable expression, termed hereditary renal adysplasia (HRA). A case of prenatal diagnosis by ultrasound of renal agenesis/dysgenesis (left renal agenesis and severe right multicystic dysplastic kidney) is described here. A woman in the 32nd week of pregnancy, who has never undergone any clinical and medical examination, presents at the ultrasound control. A male fetus was born from breech delivery at 34 weeks gestation, weighted 1950 g died after two hours because of severe pulmonary hypoplasia. Autopsy confirmed the antenatal diagnosis of left renal agenesis and right multicystic dysplastic kidney, that measured 42 x 31 x 25 mm, with bilateral complete absence of ureters and renal vessels. No other malformations were present. The infant's chromosomes were normal (46 XY). No specific chromosomal anomaly was identified in either parents. Both the parents had normal genitourinary ultrasound findings. In the wife's family pedigree, a sibling had asymptomatic unilateral renal agenesis, found at ultrasonography, and one maternal aunt had unilateral multicystic kidney and bicornuate uterus. Moreover one maternal uncle died at birth after preterm delivery from respiratory failure, unfortunately kidneys were never examined. Instead, no renal anomaly was identified in the husband's family history. The parents must be made aware not only of the inevitable fatal outcome for the fetus but also of the increased risk of recurrence in a subsequent pregnancy. On the basis of the literature, (of which this case is a further confirmation) it is underlined the necessity that all families of bilateral renal agenesis/dysgenesis patients-should have a detailed evaluation, including a detailed family history and ultrasound study of the kidneys and uterus; in fact all first-degree relatives have an increased risk of having silent genitourinary malformations.     

Use of proprioception in normal and clumsy children

This study investigates the relation between performance on simple tasks dependent on proprioception, and performance of complex perceptual-motor skills in clumsy children and age-matched control children. One hundred and forty-six right-handed children aged between 5 and 8 years were tested on non-visual aiming, non-visual posture matching, the Kinaesthetic Sensitivity Test (KST), and the Movement Assessment Battery for Children (ABC). Half of the children had scores below the 15th centile on the Movement ABC and were classed into the developmental coordination disorder (clumsiness) group. Scores on the proprioceptive tasks were used to predict performance on complex tasks of the subscales of the Movement ABC (manual dexterity, ball skills, and balance). Specific relations were found between the proprioceptive tasks and the subscales of the Movement ABC, but the KST did not predict differences in motor skills, and no relation was found between tasks carried out without vision. Simple non-visual movement tasks do predict performance in more complex skilled tasks but this is affected by many task features rather than simply the reliance on proprioception for information about movement.     

Statistical Themes and Lessons for Data Mining

Data mining is on the interface of Computer Science andStatistics, utilizing advances in both disciplines to make progressin extracting information from large databases. It is an emergingfield that has attracted much attention in a very short period oftime. This article highlights some statistical themes and lessonsthat are directly relevant to data mining and attempts to identifyopportunities where close cooperation between the statistical andcomputational communities might reasonably provide synergy forfurther progress in data analysis.     

Cocontraction changes in muscular fatigue at different levels of isometric contraction

Ten normal subjects participated in a study designed to contrast results obtained in pre- and post-fatigue states. The measures contrasted were the IEMG ratios of agonist/antagonist pairs of muscles. The experimental task was an 8 s ramp isometric elbow flexion ranging from 0 to 100% of a maximal voluntary contraction (MVC). IEMG ratios were obtained at levels of 20, 40, 60 and 80% MVC. Records from the following muscles were obtained with surface electrodes: biceps brachii (BB), brachioradialis (BR), triceps brachii (TB) and anconeus (SU). The torque at the elbow joint was measured by a Cybex II dynamometer. Fatigue was induced using a 60% MVC of elbow flexion maintained during 5 minutes. The data were collected on-line at a sampling rate of 1 kHz. The results indicated that the IEMG ratios (BB/TB and BR/SU) presented a tendency, across subjects, toward a decrease at the levels of 40%, 60% and 80% MVC at the post- relative to the pre-fatigue state. The BB/BR ratios remained stable. These changes in the post-fatigue ratios disclose a tendency toward a saturation of the agonist occurring concomitantly with an increased level of contraction of the antagonists.